A LoxP site and an FRT site flanked neomycin resistance gene cassette were inserted into intron 2 and an F3 site flanked puromycin resistance gene cassette and a second loxP site into intron 8. The neo and puro cassettes were removed through subsequent flp-mediated recombination and exons 3-8 were deleted through cre-mediated recombination. Absence of peptide expression from this allele was confirmed by western blots. (J:306481)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
Insertion, Intragenic deletion
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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