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CRISPR/cas9 genome editing is used to introduce a S153P (TCT to CCT) missense mutation, and silent mutations, including F154F (TTC to TTT) and F156F (TTT to TTC), into exon 3. The two silent changes were introduced to prevent recutting of the targeted region. The mutation is orthologous to S155P in humans and is associated with multiple sulfatase deficiency (MSD). (J:334328)