Ins2^tm2.1Jfer

Approximately 3.1 kb of upstream sequence was replaced with the equivalent region upstream of the human insulin gene but with a -331C>G mutation (relative to the translation start site) that disrupts a CC dinucleotide and that is found in some diabetes patients. IRES sequence and an EGFP reporter gene cassette were inserted into the 3' UTR. The loxP site flanked neomycin resistance gene cassette that was also inserted was removed through subsequent cre-mediated recombination. In this allele the mutated human INS promoter fails to drive expression of the mouse Ins2 gene and the EGFP reporter. (J:308553)