Spock3^{Tg(lacZ)78-8Tyama}

Using a lacZ transgene driven by the human EEF1A1/EF1alpha promoter, a transgenic mouse line (line 78-8) was established by the transgene injection into 1-cell embryos. The genomic sequencing of the transgenic mouse revealed that both Spock3 and Anxa10 were disrupted simultaneously on chromosome 8 by the transgene insertions, resulting in double-null mutations of these genes. The transgene insertion at the Spock3 was determined to be promoterless lacZ not likely to be expressed. Spock3-deficient mice were generated from the Spock3 and Anxa10 double mutants by separating two mutant loci by repeatedly backcrossing the Spock3 and Anxa10 double-mutant founder mice on a C57BL/6 C3H hybrid genetic background to the C57BL/6 strain. Two independent animals, both on a C57BL/6 genetic background, that harbor single Spock3- mutant loci separated from the Anxa10- mutant loci (Anxa10Tg(EEF1A1-lacZ)78-8Tyama) by homologous recombination were obtained. No Spock3 mRNAs were detected by in situ hybridizations in homozygous mice, confirming the null state. (J:308070)