A loxP site was inserted upstream of exon 2 and an frt-flanked neo cassette with a 3' loxP site was inserted downstream of exon 3. Cre-mediated recombination in the germline resulted in the deletion of exon 2 and the selection cassette. (J:137213)
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A loxP site was inserted upstream of exon 2 and an frt-flanked neo cassette with a 3' loxP site was inserted downstream of exon 3. Cre-mediated recombination in the germline resulted in the deletion of exon 2 and the selection cassette. (J:137213)