The targeting construct introduced an FRT site, splice acceptor, IRES sequence, lacZ reporter gene, FRT site, and loxP site. An additional loxP site was inserted downstream of exon 8. Flp-mediated recombination removed the reporter/selection cassette and left exon 8 floxed. (J:306377)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count