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The targeting construct inserted the tdTomato openreading frame into the Calca locus between just 5 of the initiation codon in exon 2 and the noncoding exon 6. An frt-flanked Sv-Neo gene was also included for positive selection. After homologous recombination in ES cells, resulting chimeras were bred with FLPase mice to remove the Sv-Neo gene. (J:301067)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
