Exon 5 was flanked by loxP sites. An FRT-flanked neomycin resistance cassette was inserted 1128 bp upstream of exon 5. Flp-mediated recombination removed the selection cassette, leaving exon 5 floxed. (J:288182)
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Exon 5 was flanked by loxP sites. An FRT-flanked neomycin resistance cassette was inserted 1128 bp upstream of exon 5. Flp-mediated recombination removed the selection cassette, leaving exon 5 floxed. (J:288182)