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CRISPR/cas9 genome editing is used to delete the mouse CH1 (constant heavy chain) exons and replace the mouse VH (variable heavy chain) portion of Igh with a VHH minigene containing: mouse Vh promoter (containing octamer and TATA box, 250 bps), mouse leader exons-intron (150 bp), camelid VHH open reading frame (300 bps), and mouse downstream sequences containing recombination sequences (100 bps). The 15 kD VHH (Variable Heavy chain domains of a Heavy chain or nanobodies [Nbs]) minigene contains 18 alpaca, 7 dromedary and 5 bactrian camel VHH genes in a 25 kB insertion. Each gene is fused to a VH promoter, leader exons and recombination signal sequences. (J:307753)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
