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By homologous recombination in the C57BL/6J ES cells, a SA-3xFLAG-NLS-SpCas9-NLS-polyA-loxP-HSVtk-Neo-loxP cassette was inserted into the Gt(ROSA)26Sor locus. Targeted ES cells were used for blastocyst injection to obtain chimeric mice. The loxP-HSVtk-Neo-loxP cassette was removed by mating with cre deleter mice. (J:293207)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
