The targeting construct introduced a loxP site 68 bp upstream of exon 1 and a floxed neomycin resistance cassette downstream of exon 1. Cre-mediated recombination removed the selection cassette and left exon 1 floxed. (J:306439)
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The targeting construct introduced a loxP site 68 bp upstream of exon 1 and a floxed neomycin resistance cassette downstream of exon 1. Cre-mediated recombination removed the selection cassette and left exon 1 floxed. (J:306439)