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A vector containing 15 kb of the endogenous genomic sequence. The tetracycline response element (TRE) element was isolated from pTre2 (Clontech) and then cloned 500bp upstream of the translation start site along with a loxP flanked neomycin resistance cassette. No endogenous gene sequences were removed or altered at the insertion site, although silent mutations were incorporated into the first exons of E2f3a and E2f3b. Finally, a DTA cassette was added to this vector to allow negative selection in mouse embryonic stem cells. Cre-mediated recombination removed the conditional neomycin resistance cassette. (J:255811)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
