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CRISPR/Cas9 genome editing is used to insert the Citrine cDNA with a linker sequence at the ATG start codon and to replace the site-directed mutagenized sequence for the oncogenic Kras protein in which the glycine at position 12 has been substituted to aspartic acid in the Kras locus so that the Citrine and oncogenic Kras G12D coding sequences are in the same reading frame. The construct replaced the LSLKrasG12D locus in frame with the KrasG12D gene in the male pronucleus of LSL-KrasG12D/+ mouse zygotes. (J:306509)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
