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The targeting vector containing Cre-ERT2-FRT-Neo-FRT-LoxP cassette inserted into exon 1 just downstream of the endogenous ATG site of Hoxa13 gene, replacing 1864 base pairs of the coding region spanning exons 1 and 2 was generated from a C57BL/6 BAC (bacterial artificial chromosome) clone (RPCI23:33N14) using homologous recombination. C57BL/6NTac 129S6/SvEvTac embryonic stem (ES) cells (Taconic) were electroporated with the targeting construct to generate chimera. After germline transmission, the Neo resistance cassette was eliminated by breeding with a Flpe deleter line. (J:301894)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
