Eomes^{tm4.1(icre)Rob}

A targeting vector introduced the coding sequence for improved Cre recombinase (iCre) coding sequence followed by a polyA cassette and a loxP-flanked neomycin-resistance cassette was introduced into the exon 1 start site of the Eomes gene, resulting in deletion of about 500 base pairs of the 5' coding region. Correctly targeted ES cells were transiently transfected with a plasmid encoding cre to remove the neomycin cassette. The resulting cells were used to generate germline chimeras. (J:306370)