Pomc^{tm1.1(cre)Lowl}

A selection cassette containing an internal ribosomal entry sequence (IRES) linked to Cre-recombinase and an Frt-flanked kanamycin resistance gene was targeted just downstream of the stop codon of the Pomc gene, so that Cre-recombinase expression was driven by the endogenous gene. Flpe recombinase transgene mice were then used to remove the kanamycin selection cassette. (J:303221)