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Homologous recombination in ES cells targeted to insert the fluorescent protein tdTomato, cre recombinase and the diphtheria toxin receptor (DTR) separated by P2A sequences (tdTomato-P2A-Cre-P2A-DTR) into the endogenous Zfp683 locus whereby the targeting sequence replaced exons 2, 3 and 4, as well as intronic sequences of the Zfp683 gene (knockin knockout). An FRT-flanked neomycin resistance gene was also introduced. Embryonic stem cell-derived offspring were then bred with FLPO mice to remove neo selection cassette. (J:306904)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
