A loxP site was inserted upstream of exon 4 and an FRT-flanked pGK-neomcyin selection cassette and a second loxP site were inserted downstream of exon 4. Flp-mediated recombination removed the selection cassette leaving exon 4 floxed. (J:306667)
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A loxP site was inserted upstream of exon 4 and an FRT-flanked pGK-neomcyin selection cassette and a second loxP site were inserted downstream of exon 4. Flp-mediated recombination removed the selection cassette leaving exon 4 floxed. (J:306667)