Foxp3^{tm4(EGFP/icre)Tch}

The targeting construct consisting of the IRES-EGFP-iCre sequence inserted immediately downstream of the Foxp3 translational stop codon and upstream of the endogenous polyadenylation signal and additional FRT flanked PGK-neo cassette inserted between exons 9-10 was used for homologous recombination in the ES cells. The PGK-neo cassette was removed by mating founder males with Flp-deleter female mice. Inserted Frt element inserted between exons 9 and 10 that created an aberrant 3 splice junction site, leading to a premature stop codon in the Foxp3 transcript. (J:305614)