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Codon changes to change phenylalanine 755 and glycine 756 to alanines (TTT>GCT (p.F755A), GGC>GCC (p.G756A)) were introduced using CRISPR/Cas9 technology with Alt-R CRISPR RNA (targeting GCCCCTGCTTGTGATCACTT), Alt-R trans-activating CRISPR RNA, high-fidelity Cas9 nuclease ribonucleoprotein and an ssODN template (ACGCCTGAAGAGCCCCCTAAACAGCGCTGCCGGGCCCCTGCTTGTGACCACGCTGCCAATGCCAAGTGTAATGGTTACTGCAATGAGTGCTACCAGTTCAAGCAGATGTATGG). This allele was generated in fertilized oocytes heterozygous for the Tnfaip3tm4.1Ama allele. Separate single-mutant for this Tnfaip3em2Ama allele and double-mutant mouse lines were subsequently established. (J:306576)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
