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CRISPR/cas9 genome editing is used to insert a CAG promoter followed by a loxP:STOP:loxP (LSL) followed by the ER-Hoxb8 fusion protein into the Gt(ROSA)26Sor locus. Transient transfection with cre recombinase removes the LSL and leaves a single loxP site. The ER-Hoxb8 fusion protein comprises the hormone binding domain of the human estrogen receptor alpha fused to the murine Hoxb8 sequence. In this system, the ER-Hoxb8 protein is constitutively expressed; the estrogen receptor fusion renders the protein functionally dependent on estrogen. (J:101977)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
