Tg(Cdhr1-tTA)#Tahi

The transgene was established using a mouse genomic BAC clone RP23-55P20, which encodes cadherin-related family member (Cdhr)1 gene by inserting a loxP-LacZ-loxP-tTA cassette at 25 bp upstream of the translation initiation site of this gene. In this cassette, the LacZ gene followed by a transcription termination site was flanked by loxP sequences and used as a STOP sequence, which followed by the tetracycline-controlled transactivator (tTA) gene in an optimized form for mammalian expression. The original loxP site in the vector backbone was subsequently deleted using p23loxZeo. Among the six independent transgenic lines obtained, one unnumbered line was characterized by the OB-specific strongest expression of the internal LacZ gene. (J:288786)