Mir205^tm1.3Mtm

A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and one loxP site immediately downstream of the microRNA stem loop. Flp-mediation removed the reporter/selection cassette and left the microRNA floxed. (J:243203)