A loxP site was inserted upstream of exon 3 and an FRT flanked neo cassette and loxP site were inserted downstream of exon 4 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exons 3 and 4 floxed. (J:260109)
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A loxP site was inserted upstream of exon 3 and an FRT flanked neo cassette and loxP site were inserted downstream of exon 4 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exons 3 and 4 floxed. (J:260109)