The targeting oligonucleotides containing loxP sites and flanking sequences of exon 3 of the gene were microinjected, along with Cas9/gRNA ribonucleoparticles, into zygotes. Cre-mediated recombination removed exon 3. (J:296556)
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The targeting oligonucleotides containing loxP sites and flanking sequences of exon 3 of the gene were microinjected, along with Cas9/gRNA ribonucleoparticles, into zygotes. Cre-mediated recombination removed exon 3. (J:296556)