A loxP site was inserted upstream of exon 8 and an FRT-flanked neomycin resistance gene cassette and a second loxP site were inserted downstream of exon 8. Flp-mediated recombination removed the neo cassette leaving exon 8 floxed. (J:289548)
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A loxP site was inserted upstream of exon 8 and an FRT-flanked neomycin resistance gene cassette and a second loxP site were inserted downstream of exon 8. Flp-mediated recombination removed the neo cassette leaving exon 8 floxed. (J:289548)