Log In|Sign Up
EN
Sequence for a Gly-Ser-Gly spacer, a OneSTrEPtag (OST), two STOP codons, an IRES2 sequence, a Kozak sequence, a sequence coding for the monomeric cyan (teal) fluorescent reporter gene mTFP1, a loxP site, and an FRT site flanked neomycin resistance gene cassette, was inserted in-frame at the 3 end of the gene coding sequence exon 19. A second loxP site was inserted into intron 18. The neo cassette was removed through subsequent Flp-mediated recombination. The fusion of the OST to the protein can be used in affinity purification. (J:239186)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
