CRISPR/cas9 endonuclease-mediated genome editing is used to insert the V2-TIR1 construct containing from 5' to 3'; a human EIF1AX (eukaryotic translation initiation factor 1A X-linked) promoter, a lox-STOP-lox cassette (LSL), the Oryza sativa TIR1 sequence with a hemagglutinin (HA) tag followed by an FRT-flanked puromycin. (J:302997)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count