A loxP site was inserted into intron 3 and an FRT site flanked neomycin resistance gene and a second loxP site into the 3' UTR in exon 4. The neo cassette was removed through subsequent flp-mediated recombination, leaving a conditional-ready allele with exon 4 floxed. (J:300667)
Basic Information
(129S6/SvEvTac x C57BL/6NCrl)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count