The genomic region containing exons 2-5 was targeted with two sgRNAs (targeting GTCAAGCATACTCCATACCC and GCGGTGTGAATTGCTGTGCC) using CRISPR/Cas9 technology, resulting in a 19773 bp deletion (deleting exons 2-5) and 2 bp insertion (CG). Western blots confirmed the absence of peptide expression from this allele. (J:271583)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Applicable
Endonuclease-mediated
Insertion, Intragenic deletion
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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