Kif9^em1Osb

CRISPR/Cas9 genome editing technology was used to generate a 16bp deletion in exon 2. The 16 bp deletion caused a P15L mutation resulting in a premature stop codon introduced three amino acids later. Western blot analysis revealed the absence of encoded protein in the testes and cauda epididymal spermatozoa from homozygous mutant male mice. Immunofluorescence confirmed the absence of protein in mutant spermatozoa. (J:303071)