Cmtm6^{tm1c(EUCOMM)Wtsi}

The L1L2_Pgk_P cassette was inserted at position 114565819 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 114569458. Exon 2 and 3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by subsequent flp-mediated recombination to remove the lacZ-neo cassette. (J:296495)