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The transgenic construct contains a CMV enhancer/chicken beta-actin (CAG) promoter, a loxP-Stop-loxP (LSL) cassette containing a green fluorescent protein sequence (EGFP) and a polyadenylation signal, followed by the ER-BioID2(HA) coding sequence. ER-BioID2(HA) is composed of an IgK signal peptide, BioID2 coding sequence (containing a mutant biotin ligase (BirA*) sequence), a hemagglutinin (HA) tag, and the endoplasmic reticulum (ER) retention signal KDEL (Lys-Asp-Glu-Leu) tetrapeptide. (J:300755)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
