CRISPR/cas9 genome editing is used to insert a T2A peptide sequence and Cre recombinase at the stop codon. T2A sequence adds EGRGSLLTCGDVEENPG to the C terminus of Adora1 and a proline to the N terminus of cre. (J:300669)
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CRISPR/cas9 genome editing is used to insert a T2A peptide sequence and Cre recombinase at the stop codon. T2A sequence adds EGRGSLLTCGDVEENPG to the C terminus of Adora1 and a proline to the N terminus of cre. (J:300669)