A loxP site was inserted into intron 1 and an FRT site flanked neomycin selection cassette and a second loxP site into intron 2. The neo cassette was removed via flp-mediated recombination in the germline, leaving a conditional-ready allele with exon 2 floxed. (J:302593)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count