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This transgene expresses GFP-LC3-RFP-LC3DeltaG, a fluorescent probe to evaluate autophagic flux, under the control of a CAG promoter. The fusion protein is cleaved by endogenous ATG4 proteases into equimolar amounts of GFP-LC3 and RFP-LC3DeltaG. GFP-LC3 is degraded by autophagy, while RFP-LC3DeltaG remains in the cytosol, serving as an internal control. Thus, autophagic flux can be estimated by calculating the GFP/RFP signal ratio. In this line, high expression was observed only in the skeletal muscle. (J:252211)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
