Tg(TcraD78,TcrbD78)#Wrh

Transgenic Plasmodium berghei ANKA strain (PbA) MHC II-restricted (H2-Ab1 (H2-IAb)) TCR (PbT-II) mice were generated using the V(D)J segments of the Tcra and Tcrb genes of a CD4+ T cell hybridoma (D78) specific for an unidentified blood-stage PbA antigen. The D78 hybridoma is derived from a T cell line isolated from a blood-stage PbA-infected C57BL/6 mouse. The Tcra chain consists of V2.7, J12, and C gene segments and the Tcrb chain of V12, D2, and J2.4. The transgene also contains the Igh chain enhancer, the H2-K1 (H2-Kb) promoter and the polyadenylation signal sequence of the human beta-globulin gene for the Tcra segments and the Tcrb enhancer, the 2B4-derived 5' region and leader sequence and the B3-derived promoter and coding regions for the Tcrb segments. (J:256756)