A loxP site was placed 1.9 kb upstream of the first exon. A second loxP site was placed in the first intron. A puromycin resistance (PuroR) gene flanked by F3 sites was inserted into intron 1 upstream of the second loxP site for positive selection. The puromycin resistance (PuroR) gene was removed via flp1-mediated recombination in the germline. (J:243813)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count