LoxP sites were inserted 190 bp upstream and 220 bp downstream of exon 2. A FRT-flanked neomycin cassette was that was inserted further downstream of exon 2 was subsequently removed via FLP1-mediated recombination in the germline. (J:128713)
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LoxP sites were inserted 190 bp upstream and 220 bp downstream of exon 2. A FRT-flanked neomycin cassette was that was inserted further downstream of exon 2 was subsequently removed via FLP1-mediated recombination in the germline. (J:128713)