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Site-specific PhiC31 integrase-mediated transgenesis is used to insert the targeting construct containing an attB site, the CAG promoter, a loxP flanked PGK-neomycin STOP cassette, a FLAG-tagged mouse Met cDNA, a polyA signal and a second attB site into the intergenic site 2 locus. This construct was microinjected, along with the PhiC31 integrase mRNA into the pronuclei of zygotes of an FVB/N mouse that contains an attP docking site at the H11 locus which is either the Igs2tm3.1Luo or the Igs2tm4Luo. The PhiC31 integrase catalyzes recombination between the attB and attP sites. (J:268934)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
