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A loxP site was inserted into intron 3 and the following elements into intron 4: an FRT site, a second loxP site, engrailed homeobox 2 (en2) intron and splice acceptor sequences, IRES sequence, the tdTomato red fluorescent protein gene with sequence for a C-terminal HA tag, poly(A) signal sequence, a neomycin resistance gene cassette, a second FRT site and a third loxP site. The sequence between the FRT sites (RFP and neo cassettes) were removed through subsequent flp-mediated recombination. Exon 4 was then deleted through cre-mediated recombination. (J:301313)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
