A loxP site was inserted 5' to exon 8 and a second loxP site and an frt-flanked neomycin selection cassettte were inserted 3' to exon 9. The neomycin selection cassette was removed via FLP-mediated recombination in the germline. (J:260660)
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A loxP site was inserted 5' to exon 8 and a second loxP site and an frt-flanked neomycin selection cassettte were inserted 3' to exon 9. The neomycin selection cassette was removed via FLP-mediated recombination in the germline. (J:260660)