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RMCE (recombinase-mediated cassette exchange) was utilized to insert tetO-GFP/RNAi:Atg5 into the Col1a1 locus. D34 embryonic stem cells, expressing CAG-rtTA3 knocked into the Rosa26 locus, were used. The vector contains a tetracycline responsive promoter (TRE;tetO) controlling expression of a GFP reporter and short-hairpin RNA sequence targeting Atg5 embedded in the miR-E design (an improved design variant of Mir30 structure). (J:289254)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
