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CRISPR/cas9 genome editing is used to insert loxP sites on either side of exon 4. A double stranded oligonucleotide donor DNA with a 1.6-kb left arm, a 1.1-kb right arm, two loxP sequence elements [with the sequence ATAACTTCGTATAGCATACATTATACGAAGTTAT] and all of exon 4 is used to generate the floxed allele. The 5' loxP sequence is positioned 211nt 5' of exon 4 and the 3' loxP sequence is positioned 170nt 3' of exon 4. Progeny were screened by DNA sequencing to identify correctly targeted pups. (J:101977)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
