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The targeting construct was designed to fuse a P2A self-cleaving peptide sequence and a cre/ERT2 sequence in frame at the 3' of exon 14 of the retinal pigment epithelium 65 (Rpe65) gene. The cre-ERT2 fusion gene contains a Cre recombinase fused to a human estrogen receptor ligand binding domain. A frt-flanked neomycin resistance (neo) cassette was placed immediately after the non-coding exon 15. This construct was electroporated into C57BL/6 FLP-derived iTL BF1 embryonic stem (ES) cells. The Neo cassette in the targeting sequence was removed during ES clone expansion. (J:310003)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
