The GFP fluorescent marker gene and linker sequence was inserted upstream of the coding region in exon 3 using a donor plasmid, a crRNA (CAGAGGAUGAGCGAACCACAGUUUUAGAGCUAUGCUGUUUUG) and a tracrRNA (AAACAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCU) with CRISPR/Cas9 technology. (J:291079)