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Exon 1 was targeted with sgRNAs (TAGGCTCTCTGCAATGGCTGAC, AAACGTCAGCCATTGCAGAGAG, TAGGGGTAAGCAGGTTGCTGA and AAACCTCAGCAACCTGCTTACC) and a donor plasmid containing a GFP gene and poly(A) signal sequence using CRISPR/Cas9 technology. This resulted an a knockout reporter allele where exon 1 is replaced with the GFP gene. (J:293325)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
