LoxP sites were inserted into introns 1 and 3. An FRT site flanked neomycin resistance gene cassette was inserted between exon 3 and the loxP site. Cre-mediated recombination deleted the neo cassette and left exons 2 and 3 floxed. (J:239950)
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LoxP sites were inserted into introns 1 and 3. An FRT site flanked neomycin resistance gene cassette was inserted between exon 3 and the loxP site. Cre-mediated recombination deleted the neo cassette and left exons 2 and 3 floxed. (J:239950)