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CRISPR/Cas9 technology was used to flank exons 3 and 4 with loxP sites and inserted an FRT-flanked neomycin selection cassette downstream of exon 4 but upstream of the second loxP site. Cre-mediated recombination removed exons 3 and 4 and the neo cassette resulting in a frame-shift. Western blotting confirmed absence of protein in central nervous system tissue and sciatic nerve. (J:295996)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
