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The targeting construct includes a Tet-responsive element (TRE or tetO) directing expression of an eGFP reporter followed by an IRES and a truncated (amino acids 1-312) human protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1 or AMPKalpha;1) cDNA. The construct, a "Flp-In" promoter cassette (PGK-promoter-ATG-FRT) and a pCAGGS-FlpE plasmid were electroporated into modified (C57BL/6 x 129S4/SvJae)F1-derived D34 embryonic stem (ES) cells. (J:278318)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
